Hematological Parameters: Manual vs Automated Method; Among the β-Thalassemia and other Haemoglobinopathies in a Tertiary Care Hospital in Kolkata

Background: According to a report of WHO in 2007, 7% world populations are carrier for Haemoglobindisorder and accurate timely detection various Hb variants including beta thalassemia trait can preventoccurrence more serious disorders like major new-borns. But developing country ususe high pressure liquid chromatography (HPLC) is limited, manual testing done assessmentis quite impossible. So, an observational cross-sectional study was among 117 β-thalassemia otherHemoglobinopathies cases carriers.Objective: To find out the variation between results haematological tests obtained by automated countingchamber method.Materials & Methods: This Observational, at Thalassemia control unit Physiologydepartment R G Kar Medical College Hospital, Kolkata. other Hemoglobinopathies casesand carriers detected complete blood count with HPLC from Outpatient Department (OPD) andantenatal mothers antenatal clinic (ANC) were population this study. Findings variations thefeatures different hematological parameters amongst hemoglobinopathies werereviewed. A finding HbA, HbA2, HbF, HbD, HbE.Results: Result only Hb% method significantly inβ- THALASSEMIA Trait (p=0.0001), HbE HbS (p=0.0001) not significant diseaseand no Red cell indices two methods. Conclusion: As featureslike TC RBC or PCV shows differences HPLC/Automated counter assessment, such methods be utilized even primary level cost effectively assessment hematologicaldisorders. difference seen estimating Hemoglobin percentage Shalli‘s andHPLC/Automated assessment. So some alternate may introduce lower health systemother than Shalli’s